Microscopy | Photonics.com

MICROSCOPY DICTIONARY TERMS

Light Sheet Fluorescence Microscopy (LSFM)Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and fast biological processes in vivo. In this method, a light sheet illuminates the specimen from the side in a single focal plane...

epifluorescence microscopyFluorescence imaging technique in which the excitation light from the objective is directed into the sample producing...

bright-field illuminationThe illumination generally used in microscopy, whereby the specimen appears dark against a light background.

fiber-based confocal luminescence microscopeA microscope in which laser light is delivered through single-mode fibers that replace the pinhole usually used in confocal...

high-content screeningAlso known as HCS, an analytical method designed to collect statistically relevant amounts of quantitative data on many...

two-photon excited fluorescenceTwo-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living...

differential interference contrast microscopyDifferential interference contrast (DIC) microscopy, sometimes called Nomarski Microscopy after the inventor Georges...

laser microscopyTechnique using functional optical microscope with the addition of a coherent source collinear with the image path. The...

morphological processingA set of algorithms used to study the structural and spatial characteristics of objects in a digitized image for...

second-harmonic generation microscopyA nonlinear label-free imaging technique commonly used during surgical procedures for the visualization of collagen fibers...

chemical microscopyThe field of microscopy as applied to chemical problems and analysis.

StereomicroscopyThe use of a specialized optical microscope designed to provide a more three-dimensional view of a sample. Stereomicroscopy...

ultraviolet microscopeA microscope used either to detect selective absorption of various wavelengths by the specimen or to achieve increased...

Nomarski microscopyAlso referred to as differential interference contrast (DIC) microscopy; Nomarski microscopy is a unique form of microscopy...

microscopyThe technology of the interpretive use and applications of microscopes.

reflection x-ray microscopyA means of high resolution study through the application of soft and hard x-rays onto a sample surface in order to obtain...

wide-field fluorescence microscopyWide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are...

cardioid condenserAn oil immersion condenser used to permit only light that has been diffracted or dispersed by a microscope specimen to enter...

optical stainingWith respect to microscopy, also called dispersive staining, optical staining the projection of colors through areas of a...

magnetic lensAn arranged series of coils, magnets or electromagnets disposed in such a way that the resulting magnetic fields generate a...

beta fluorographyThe use of a short-duration electron beam to record high-speed events that occur in microscopic objects made of materials...

tip-enhanced Raman spectroscopyA spectroscopic technique that combines the high chemical specificity of Raman scattering and signal sensitivity provided by...

holographic microscopyThe holographic recording of a microscopic specimen whereby magnification is achieved by alteration in a wavelength or...

quantum cascade laserA Quantum Cascade Laser (QCL) is a type of semiconductor laser that emits light in the mid- to far-infrared portion of the...

working distanceIn microscopy, the clear distance between the specimen being viewed and the first optical element of the objective lens.

photoelectron microscopySurface analysis by means of photon induced electron emission. PEM methods provide high lateral resolution of the observed...

phase-contrast generationMicroscopy technique to convert the phase structure of the wave transmitted or reflected by the specimen into a...

microscope immersion fluidThe liquid used in microscopy to fill the space between the high-power objective lens and the microscope slide in order to...

ultraviolet microscopyThe study and photographing of microscope specimens in ultraviolet light; using an optical microscope containing fluorite...

oblique illuminationA common technique in microscopy, oblique illumination is one in which the object is illuminated by a light source that is...

direct illuminationLight produced by visible radiation that moves from the light source to the object without reflection. With respect to...

vertical incident illuminationWith respect to microscopy, light that is directed down through the objective onto the specimen and then returned by...

point-projection x-ray microscopyA method of producing magnified images by x-rays. The specimen is placed close to a point source of x-rays; the...

indirect illuminationThe light formed by visible radiation that, in traveling from light source to object, undergoes one or more reflections. In...

random access multiphoton microscopyAlso known as RAMP microscopy, random access multiphoton microscopy is a microscopic technique that uses multiple...

vertical transmitted illuminationWith respect to microscopy, light that is directed through the specimen by a substage condenser.

X-ray Phase Contrast Microscopy (XRIM)Used for high-resolution surface study with subnanometer resolution. XRIM uses interfacial phase contrast with application...

scanning coherent slope microscopyMeasures by heterodyning interferometry the local slope of a vibrating sample. The method allows the reconstruction of a...

dark-field microscopyA technique whereby the sample is illuminated by a hollow cone of light larger than the acceptance angle of the objective,...

counting chamberIn microscopy, the chamber that is contained on a microscope slide to hold a certain amount of fluid. It is calibrated...

interphako interference microscopyMeasures the refractive indices axially from the fiber profile. Microscopy technique provides an interferogram with high...

feret's diameterIn microscopy, the measured distance between theoretical parallel lines that are drawn tangent to the particle profile and...

Golgi Staining TechniqueThe Golgi staining technique (also called the black reaction) makes it possible to look at the nervous system by rendering...

fluorescence microscopyObservation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane...

zero-order filteringThe removal of the zero-order component of the Fourier spectrum distribution of an object with a small, opaque absorber or...

carbon filmIn analysis, the carbon layer that is evaporation-deposited on a specimen to protect and ready it for study by electron...

stage micrometerIn microscopy, a calibrated scale on a slide that may be viewed to determine the exact magnification factor of the...

diffrimicroscopy

video-intensified microscopy (VIM)The observation of specimens by a microscope linked to a television camera and an image processor.

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